Effect of Tubiechong-Containing Serum on Angiogenesis in Chick Chorioallantoic Membrane

Objective To research the possible anti-neoplastic mechanism of Tubiechong( Eupolyphaga Seu Steleophaga). Methods Totally 56 chick embryos were prepared into the chick chorioallantoic membrane( CAM)models and randomized into the blank group and Tubiechong-containing serum high-dose,middle-dose and low-dose groups. The blank group was given no treatment. The Tubiechong-containing serum high-dose,middle-dose and lowdose groups were given 11 g /kg·d,8 g /kg·d and 5. 5 g /kg·d Tubiechong-containing serum respectively. The incubation was continued for 72 hours. The survival of chick embryos,morphology of CAM models and angiogenesis of CAM models were observed. Results The survival rate of chick embryos in each group was same. The embryo was active,CAM was transparent and vascular was clear and red in survival chick. The vascular was lighter and unclear and embryo was inactive in the dead chick. Comparing with the blank group,the angiogenesis in the Tubiechong-containing serum high-dose,middle-dose and low-dose groups were significantly decreased( P 0. 05). Comparing with the Tubiechong-containing serum middle-dose and low-dose groups,the angiogenesis in the Tubiechong-containing serum high-dose group was significantly decreased( P 0. 05). There was no significant difference between the Tubiechongcontaining serum middle-dose and low-dose groups in the angiogenesis( P 0. 05). Conclusion The anti-neoplastic effect of Tubiechong may be related to inhibiting the angiogenesis of CAM,with a better effect of large dose.