Experimental Research of Angiogenenic Effects of TanshinoneIIA on the Chick Embryo Chorioallantoic Membrane(CAM)Model

Objective To investigate the angiogenenic effects of Tanshinone ⅡA(TsⅡA)on the chick embryo chorioallantoic membrane(CAM).Methods 42 chick embryo(CE)were randomly divided into 6 groups:7 CE in group A were treated with normal saline as negative controls;7 CE in group B were treated with menstruum;7 CE in group C were treated with basic fibroblast growth factor(bFGF)as positive controls;21 CE in group D,E and F were treated with different dose of TsⅡA respectively.After 3 days of incubation,the first and second grade vessels of CAM were counted respectively.Results The proliferation of vessels in experimental groups was more obvious than that in physiological saline group and menstruum group(P0.01 or P0.05),and had a certain dose dependent relationship.The proliferation of vessels in TsⅡA 0.010 mol/L group was more obvious than that in TsⅡA 0.005 mol/L group(P0.05),but difference between TsⅡA 0.015 mol/L group and TsⅡA 0.010 mol/L group was no significant(P0.05).Conclusion TsⅡA can promote angiogenesis of the chick embryo chorioallantoic membrane.