Effects of arctigenin on angiogenesis of chick embryo chorioallantoic membrane

Objective To observe the influence of arctigenin on the angiogenesis of chick embryo chorioallantoic membrane( CAM). Methods Arctigenin was prepared by the procedure of ethanol crystallization from Fructus Arctii and we used the windowing method to prepare the CAM model. Forty chick embryos of CAM models were randomly divided into the negative control group,low( 2. 5 g /L),medium( 5. 0 g /L),and high( 10. 0 g /L) doses of arctigenin groups,10 embryos in each group. Then the negative control solution containing DMSO and each dose of liquid containing arctigenin were added respectively onto the surface of the carrier CAM. The embryo CAM model was prepared after 8-day incubation. CAM sample was cut to observe the serum angiogenesis in the drug carrier area,to count the number of new blood vessels,and to observe the levels of new blood vessels under dissecting microscope,meanwhile,the number of new blood vessels in each drug groups within 5 mm of carrier edge was recorded,and vascular inhibition rate( IR) was calculated. Results The angiogenesis of CAM in low,medium and high doses of arctigenin groups was inhibited,the 3 +,4 + number of the medium and high doses of arctigenin groups was more than that of the negative control group( P 0. 05). The numbers of new blood vessels in the negative control group,high dose,medium dose and low dose group were respectively( 78. 26 ±5. 62),( 59. 22 ± 4. 16),( 37. 43 ± 6. 51) and( 24. 17 ± 3. 23). The neovascularization number of low dose group was less than that of the negative control group( P 0. 05),medium and high dose groups were less than that of the negative control group( P 0. 01) and that of the low dose group( P 0. 05). The neovascular IR of low,medium and high dose groups were 24. 33%,52. 17% and 69. 12%,respectively. Conclusion Arctigenin can inhibit the angiogenesis of CAM.