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2013•Chinese Journal of Spectroscopy LaboratoryRequires access

Determination of the Content of Puerarin in Xiaoke Pill by TLC Scanning

Cheng Geng

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Abstract

A TLCS method was used to determine the content of puerarin in Xiaoke Pill.The adsorbent was silica GF254,the developer consisted of chloroform-methanol-ethyl acetate-water (3∶3∶4∶1) and the determination wavelength was 254nm.The results revealed that the regression equation of puerarin wasy=1064x+16290(r=0.9949),which was linear over the range of 2.0—10.0μg.The average recovery was 101.1%,RSD=1.7%(n=5).This method is simple and accurate with good repeatability.It was a suitable method in measuring the content of puerarin in Xiaoke Pill.

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What this paper is about

A TLCS method was used to determine the content of puerarin in Xiaoke Pill.The adsorbent was silica GF254,the developer consisted of chloroform-methanol-ethyl acetate-water (3∶3∶4∶1) and the determination wavelength was 254nm.The results revealed that the regression equation of puerarin wasy=1064x+16290(r=0.9949),which was linear over the range of 2.0—10.0μg.The average recovery was 101.1%,RSD=1.7%(n=5).This method is simple and accurate with good repeatability.It was a suitable method in measuring the content of puerarin in Xiaoke Pill.

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Available abstract

A TLCS method was used to determine the content of puerarin in Xiaoke Pill.The adsorbent was silica GF254,the developer consisted of chloroform-methanol-ethyl acetate-water (3∶3∶4∶1) and the determination wavelength was 254nm.The results revealed that the regression equation of puerarin wasy=1064x+16290(r=0.9949),which was linear over the range of 2.0—10.0μg.The average recovery was 101.1%,RSD=1.7%(n=5).This method is simple and accurate with good repeatability.It was a suitable method in measuring the content of puerarin in Xiaoke Pill.

Key concepts: Puerarin, Chromatography, Repeatability, Chloroform, Chemistry, Ethyl acetate, Content determination, Pill

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