[Determination of scopolamine and atropine in Flos Daturae by RP-HPLC].

OBJECTIVE: To develop a quantitative analytical procedure of scopolamine and atropine in Flos Daturae using RP-HPLC. METHOD: The two alkaloids were separated on a Hypersil BDS C18 column (4.6 mm x 250 mm, 5 microm) with a mobile phase of 0.02 mol x L(-1) sodium acetate buffer (containing 0.02% triethanolamine and the pH was adjusted to 6.0 with acetic acid)-methanol (60:40) and a detection wavelength of 215 nm. The flow rate was 1.0 mL x min(-1) and the column temperature was maintained at room temperature. RESULT: The mean recovery was (99.6 +/- 1.8)% for scopolamine and (100.4 +/- 1.5)% for atropine. CONCLUSION: This method was simple, accurate and sensitive.