Qualitative and Quantitative Research of the Tropane Alkaloids in Inbelladonna Mixture

OBJECTIVE To establish a method for qualitative and quantitative determination of the trapane alkaloids in Inbelladonna mixtuxe. METHODS RP-HPLC methods was adopted for the atropine,scopolamine and anisodamine identification and the content of Atropine sulfate was also determined. The analyses were performed on a ZORBAX SB-C18( 4. 6 × 150 mm,5μm) column with the mobile phase consisted of acetonitrile-phosphate buffer( p H2. 8)( 13∶ 87) at a flow rate of 1. 0m L·min- 1,the detection wavelength was 210 nm,the temperature of column was30℃,and the injection volume was 20μL. RESULTS The chromatogram of the sample solution showed similarity to the reference solution. The linear range of Atropine sulfate was in the range of 15. 06 ~ 120. 5μg · m L- 1( r =0. 9998),the average recovery( n = 9) was 98. 27% and the RSD was 0. 85%. CONCLUSION The method is accurate,reliable and specific,which can be used for the quality contorl of Belladonna mixture.