Determination of scopolamine and atropine in different parts of Przewalslia tangtuica Maxim by HPLC

OBJECTIVE To develop a quantitative analytical methods of scopolamine and atropine in different parts of Przewalslia tangtuica Maxim using RP-HPLC.METHODS The content of galic acid was detected by HPLC.The separation was carried out on a Shimadzu VP C18 column(250 mm × 4.6 mm,5 μm).The mobile phase was methanol-0.02 mol·L -1sodium acetate buffer (30∶70,containing 0.1% triethanolamine and 0.3% Tetrahydrofuran and the pH was adjusted to 6.48 with acetic acid).The flow rate was 1.0 mL·min -1.The detective wavelength was set at 215 nm.The column temperature was 30℃.RESULTS The calibration curve of scopolamine and atropine showed good linearity over the range of 0.068-0.408 μg (r = 0.9996)and 2.498-14.988 μg (r = 0.9999).CONCLUSION This method was simple,accurate and sensitive.