Callus Induction and Plantlet Regeneration of Kentucky Bluegrass(Poa pratensis L.)Ganlanqiu 2

Effect of different culture medium and growth regulator on callus induction, proliferation, seedling differentiation and plant regeneration were studied by using the seeding of Kentucky Bluegrass(Poa pratensis L.) Ganlanqiu 2 as explant. The results showed that, adding 10 mg/L Ag NO3 to induction medium inhibited the growth of leaf bud and enhanced the callus induction; the callus induction frequency of HNP2 medium was apparently higher than that of HNP3 medium, indicating the medium component of HNP2 was suitable for Ganlanqiu 2; N19, N21 and N22medium adding with appropriate concentration of 6- BA, TDZ should be adopt for callus propagation; when callus was cultured on differentiation medium R1 after subculturing for 1- 2 cycles, the differentiation rate and regeneration frequency were 78.4% and 30%- 50%, respectively, providing a good acceptor system for future transformation of resistance gene.