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Callus Induction and Establishment of the Plant Regeneration System of Kentucky bluegrass.

Ding Luming, Long Rui-jun, Changting Wang

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Abstract

The main factors which influence the callus induction, differentiation, rooting and transplant of Kentucky bluegrass (Poa pratensis L.) from mature seeds were studied. The plant regeneration system has been established. The results showed that the callus inducing ratio reached 74.9±7.35% on the MS medium containing 3.0 mg/L 2,4-D and 0.1mg/L 6-BA. 8.1±0.36 sprouts per callus could be obtained in SH medium containing 0.1mg/L 2,4-D. The optimum condition for rooting was 1/2MS+1.0mg/L NAA.

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The main factors which influence the callus induction, differentiation, rooting and transplant of Kentucky bluegrass (Poa pratensis L.) from mature seeds were studied. The plant regeneration system has been established. The results showed that the callus inducing ratio reached 74.9±7.35% on the MS medium containing 3.0 mg/L 2,4-D and 0.1mg/L 6-BA. 8.1±0.36 sprouts per callus could be obtained in SH medium containing 0.1mg/L 2,4-D. The optimum condition for rooting was 1/2MS+1.0mg/L NAA.

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Available abstract

The main factors which influence the callus induction, differentiation, rooting and transplant of Kentucky bluegrass (Poa pratensis L.) from mature seeds were studied. The plant regeneration system has been established. The results showed that the callus inducing ratio reached 74.9±7.35% on the MS medium containing 3.0 mg/L 2,4-D and 0.1mg/L 6-BA. 8.1±0.36 sprouts per callus could be obtained in SH medium containing 0.1mg/L 2,4-D. The optimum condition for rooting was 1/2MS+1.0mg/L NAA.

Key concepts: Callus, Poa pratensis, Regeneration (biology), Botany, Biology, Horticulture, Chemistry, Poaceae

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