A Study on the Technique of Plant Regeneration from Mature Seed Embryo of Kentucky bluegrass in vitro

Mature seeds of Poa pratensis L.cv. Daqingshan and Total eclipse were used for explants. Effect of hormone compositions of different kinds and ration on callus induction, growth state and shoot regeneration was studied. Results indicated that the frequency of callus induction reached 30%～43% in the callus induction medium supplemented with 2.0 mg/L 2,4-D and 0.2 mg/L BA. During subcultures, rapid growth of callus and embryogenesis were maintained in the callus subculture medium plus 1.0 mg/L 2,4-D and 0.5 mg/L BA. After callus subcultures, the rate of shoot regeneration from callus was about 30% in the differentiation medium supplemented with 2.0 mg/L BA or CPPU. Selecting the callus of dry, compact, small pellet was a simple effective method in early generation to overcome the obstruction of plant regeneration.