Effect of Ensoplasmic Reticulum Stress-Relative Molecular Chaperone ERdj1 on the Pathogenesis of Preeclampsia

Objective:To evaluate the relationship between the pathogenesis of preeclampsia (PE) and the mRNA and protein expression of ERdj1(ER DnaJ1).Methods:36 pregnant women were recruited in this study,including 12 normal pregnant women as control group,12 pregnant women with mild PE and 12 pregnant women with severe PE.Electron Microscopy was used to measure ultrastructure changes of the endoplasmic reticulum in placenta trophocytes.Reverse transcription(RT) PCR and Western blot were used to investigate the mRNA and protein expression of ERdj1 in placenta.Results:①There was no swelling or expansion of endoplasmic reticulum in control group,while there were expansion,vacuolation and the increase of the volume of endoplasmic reticulum in mild and severe PE group.②The mRNA expression of ERdj1 in mild and severe PE group(2.1327±0.4855,2.3064±0.1004) were significantly higher than that of control group(0.2809±0.0064,P0.01).Also there was a significant difference of the ERdj1 mRNA between the mild and severe PE group(P0.05).③The protein expression of ERdj1 in mild and severe PE group(3.8412± 0.8423,4.9421±0.9810) were significantly higher than that of control group(1.5678±0.2315,P0.01),also this protein level in severe PE group was markedly different from that in the mild PE group(P0.05).Conclusions:There is the obvious expansion and swelling of the endoplasmic reticulum in the placenta of preeclampsia,also the endoplasmic reticulum stress-relative molecular chaperone ERdj1 may be involved in the pathophysiological processes of preeclampsia.