Expression of receptor of advanced glycation end products and P-selectin in placenta of preeclampsia patients

Aim:To explore the role of receptor of advanced glycation end products(RAGE) and P-selectin in the development of preeclampsia(PE).Methods:SP method and RT-PCR analysis were used to detect the expression of RAGE and P-selectin protein and mRNA in placenta tissue from 40 cases of PE(20 cases of mild PE,20 cases of severe PE) and 30 cases of normal term pregnant.Results:All groups showed RAGE and P-selectin positive pigmentation in the placenta.RAGE protein was mainly expressed in trophoblast cells,but there was a small amount in the vascular endothelial cells.P-selectin was mainly expressed in syncytiotrophoblast cells,vascular endothelial cells,and interstitial cells.There were significant differences among three groups on the expression of RAGE and P-selectin protein(F=250.152 and 274.044,P0.001) and mRNA(F=624.550 and 708.351,P0.001).Expression of RAGE and P-selectin mRNA was significantly higher in placenta tissue of PE group compared with that of control group(P0.05),the levels of severe PE group were higher than those of mild PE group(P0.05),and there was a positive correlation between them of protein and mRNA expression(r=0.814 and 0.796,P0.001).Conclusion:The high levels of RAGE and P-selectin in placenta tiusse in PE may be associated with the occurrence and development of PE,and both of them have synergies in the development of PE.