ScholarLens Back to search
2010•China Animal Husbandry & Veterinary MedicineRequires access

Cloning and Sequencing of NS2 Gene of Muscovy Duck Parvovirus

JI Yan-ju

Open publisher page 0 citations

Abstract

According to the sequence of MDPVFM strain published in GenBank,a pair of primers was designed by Primer 5.0.NS2 gene of MDPV and amplified by PCR.The gene of interest was cloned into the vector pMD18-T and sequenced.The results showed that NS2 gene consisted of 1357 nucleotides and encoded 451 amino acids.The NS2 gene shared 99.26% and 98.23% homology with that of FM isolate at nucleotide and amino acid level,respectively.

About this research paper

What this paper is about

According to the sequence of MDPVFM strain published in GenBank,a pair of primers was designed by Primer 5.0.NS2 gene of MDPV and amplified by PCR.The gene of interest was cloned into the vector pMD18-T and sequenced.The results showed that NS2 gene consisted of 1357 nucleotides and encoded 451 amino acids.The NS2 gene shared 99.26% and 98.23% homology with that of FM isolate at nucleotide and amino acid level,respectively.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

According to the sequence of MDPVFM strain published in GenBank,a pair of primers was designed by Primer 5.0.NS2 gene of MDPV and amplified by PCR.The gene of interest was cloned into the vector pMD18-T and sequenced.The results showed that NS2 gene consisted of 1357 nucleotides and encoded 451 amino acids.The NS2 gene shared 99.26% and 98.23% homology with that of FM isolate at nucleotide and amino acid level,respectively.

Key concepts: GenBank, Gene, Cloning (programming), Homology (biology), Biology, Primer (cosmetics), Genetics, Amino acid

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Cloning and Sequencing of NS2 Gene of Muscovy Duck Parvovirus — Research Paper | ScholarLens