2003•Zhongguo yufang shouyi xuebaoRequires access
Cloning and sequencing of NS2 gene of goose parvovirus
Xing Ming
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Abstract
According to the sequence of GPV B strain published in GenBank, a pair of primers were designed by Oligo4.0.NS2 gene of GVP and amplified by PCR. The gene of interest was cloned into the vector pMD18_T and sequenced. The results showed that NS2 gene consisted of 1356 nucleotides and encoded 451 amino acids. The NS2 gene shared 98.75 % and 98.67 % homology with that of B isolate at nucleotide and amino acid level, respectively.