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2003•Zhongguo shouyi ke-jiRequires access

Cloning and sequence analysis of VP1 gene of goose parvovirus

Baoquan Liu

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Abstract

A pair of primers was designed to amplify VP1 gene of GPV by PCR according to the published sequence of GPV B strain in GenBank. The product of PCR whose length is 2.2 kb was cloned to the pMD 18-T vector,then made sequencing and analyzing . The result of sequence showed that VP1 gene (included) 2 199 bp, which encoded 732 amino acids. The gene shared 98.50% and (93.18%) homology in (bases), and shared 98.09% and 95.77% respectively in amino acid with GPV strains B and YG.

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What this paper is about

A pair of primers was designed to amplify VP1 gene of GPV by PCR according to the published sequence of GPV B strain in GenBank. The product of PCR whose length is 2.2 kb was cloned to the pMD 18-T vector,then made sequencing and analyzing . The result of sequence showed that VP1 gene (included) 2 199 bp, which encoded 732 amino acids. The gene shared 98.50% and (93.18%) homology in (bases), and shared 98.09% and 95.77% respectively in amino acid with GPV strains B and YG.

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Available abstract

A pair of primers was designed to amplify VP1 gene of GPV by PCR according to the published sequence of GPV B strain in GenBank. The product of PCR whose length is 2.2 kb was cloned to the pMD 18-T vector,then made sequencing and analyzing . The result of sequence showed that VP1 gene (included) 2 199 bp, which encoded 732 amino acids. The gene shared 98.50% and (93.18%) homology in (bases), and shared 98.09% and 95.77% respectively in amino acid with GPV strains B and YG.

Key concepts: Biology, GenBank, Gene, Cloning (programming), Genetics, Homology (biology), Sequence analysis, Sequence (biology)

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