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2010•Unpublished venueRequires access

Cloning and Sequence Analysis of B2L Gene of Orf Virus Isolated from Jilin Province

Huyan Han

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Abstract

According to the DNA sequence of Orf virus which was published on Genbank,two pairs of primers have been designed to amplify B2L gene of JS04 by PCR.The amplified production was combined with pMD19-T vector,then was transformed into DH5.Positive clones identified correctly were sequenced.The length of B2L gene was 1137bp,and the corresponding amino acid sequence was conducted.Sequence analysis showed that the B2L gene homology among ORFNZ2,Shanhjahnpur82/04 strains was 97.8%～ 99.5%.The amino acid sequence homology was 97.6%～ 99.5%.The analysis result of phylogenetic tree showed that the B2L gene.

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What this paper is about

According to the DNA sequence of Orf virus which was published on Genbank,two pairs of primers have been designed to amplify B2L gene of JS04 by PCR.The amplified production was combined with pMD19-T vector,then was transformed into DH5.Positive clones identified correctly were sequenced.The length of B2L gene was 1137bp,and the corresponding amino acid sequence was conducted.Sequence analysis showed that the B2L gene homology among ORFNZ2,Shanhjahnpur82/04 strains was 97.8%～ 99.5%.The amino acid sequence homology was 97.6%～ 99.5%.The analysis result of phylogenetic tree showed that the B2L gene.

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Available abstract

According to the DNA sequence of Orf virus which was published on Genbank,two pairs of primers have been designed to amplify B2L gene of JS04 by PCR.The amplified production was combined with pMD19-T vector,then was transformed into DH5.Positive clones identified correctly were sequenced.The length of B2L gene was 1137bp,and the corresponding amino acid sequence was conducted.Sequence analysis showed that the B2L gene homology among ORFNZ2,Shanhjahnpur82/04 strains was 97.8%～ 99.5%.The amino acid sequence homology was 97.6%～ 99.5%.The analysis result of phylogenetic tree showed that the B2L gene.

Key concepts: GenBank, Gene, Phylogenetic tree, Sequence analysis, Biology, Genetics, Homology (biology), Cloning (programming)

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