Optimization of Conditions of Hydrolysis Decapterus Maruadsi with Protamex Protease

The effects of the main parameters including hydrolysis temperature,pH value,hydrolysis time were investigated with Single Factor Experiment and dosage of enzyme and protein concentration were investigated with response surface methodology(RSM).The optimized conditions are:concentration of protamex protease is 2 700 U·g-1 protein,protein concentration is 1.56%,hydrolysis temperature is 60 ℃,hydrolysis pH value is 7.5,hydrolysis time is 8 hours.Under the conditions,amino acid nitrogen concentration attains 1.27 mg·mL-1,hydrolysis degree attains 50.98% and total volatile basic nitrogen reaches 0.032 mg·mL-1.