Extract Flavonoids of Hawthorn by Ultrahigh Pressure

The ultrahigh pressure extraction method was used to extract flavonoids from Hawthorn at normal temperature.The content of flavonoids was determined by UV-spectrophotometry.Parameters that may influence the extraction process were investigated.The orthogonal test was applied to obtain the optimum extraction condition,which was as follows:solvent of 50% ethanol,ratio of raw Hawthorn(g) to solvent(mL) of 1:40,marinate time of 2 hours,extraction pressure of 300mPa and extraction time of 3 min.Results of this work show that the ultrahigh pressure extraction method has several advantages,such as shorter extraction time,and less impurity in the extracted flavonoids.