Analysis of Autoantibody Profiles of 374 Cases of Systemic Lupus Erythematosus

Objective To explore the diagnostic value and clinical significance of autoantibody test for SLE patients. Methods Serum samples of 374 SLE patients were analyzed by detecting antinuclear antibody( ANA),anti-ds-DNA antidody and ENA antibody repertoire( anti-Sm,anti-RNP,anti-SS-A,anti-SS-B,antiScl70 and anti-Jo1 antibody) with indirect immunofluorescence assay( IIFA) and gold linked immunodotting percolation and enzyme linked immunodotting respectively. Results ①Among the 374 SLE patients,there were 31 cases of ANA negative of IIFA test,and the positive rate of ANA( IIFA) for SLE patients was91. 7%,and speckled pattern and speckled / homogeneuous pattern were the major immunofluorescence patterns; ②the positive rate of anti-Sm antibody,anti-RNP antibody,anti-SS-A antibody,anti-SS-B antibody,anti-Scl70 antibody,anti-Jo1 antibody and anti-dsDNA were 15. 0%,23. 8%,50. 5%,12. 0%,0%,0. 3%and 43. 8%. Conclusion Co-detection has great clinical significance for diagnosis and evaluation of curative effect of SLE.