Analysis of children rotavirus diarrhea in Lanzhou in 2009

Objective:To study the epidemiological status on rotavirus diarrhea among children aged 0～5 years old in Lanzhou in 2009,and provide the epidemic and variation data of rotavirus strain for the development of rotavirus vaccine.Methods:313 stool specimens were collected from the First Hospital of Lanzhou University,and were detected by ELISA Kits.Serotype G and genotype P identifications of the rotavirus were determined by RT-PCR.Results:RV was detected in 159 of 313(50.80%) specimens.RV diarrhea admissions peaked between October and December.G3(51.57%) was the predominant strain followed by G1(7.55%)、G2(6.29%) and G9(5.66%).The most common G-P type combinations were P[8]G3(33.82%),P[4]G3(19.12%),P[4]G2(5.88%),P[8]G1(1.47%),and other uncommon strains of the type were P[6]G3,P[6]G9,P[4]G9 and P[8]G9.Conclusion:RV was a major pathogen of diarrhea in children aged 0～5 years old in Lanzhou,and the virus variation of the epidemical strains were multiplex.