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2007•Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access

HPLC Method for the Determination of Baicalin in Julan Granules

Li Jun

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Abstract

Aim To develop an HPLC method for determination of the content of Baicalin in Julan Granules.Methods A Waters:Symmetry C18 column(3.9mm×150mm,5μm) was used served as the chromatographic column.The mobile phase consisted of methanol-water-phosphate acid(47∶53∶0.2).The UV detection wavelength was 280nm.The flow rate was 1.0ml/min.Results The standard curve was linear in the range of 0.08~0.72μg,r=0.999 6.The average recovery was 98.76%,RSD=1.52%(n=9).Conlusion The method is simple,effective and accurate, and can be used for quality control of Julan Granules.

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Aim To develop an HPLC method for determination of the content of Baicalin in Julan Granules.Methods A Waters:Symmetry C18 column(3.9mm×150mm,5μm) was used served as the chromatographic column.The mobile phase consisted of methanol-water-phosphate acid(47∶53∶0.2).The UV detection wavelength was 280nm.The flow rate was 1.0ml/min.Results The standard curve was linear in the range of 0.08~0.72μg,r=0.999 6.The average recovery was 98.76%,RSD=1.52%(n=9).Conlusion The method is simple,effective and accurate, and can be used for quality control of Julan Granules.

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Available abstract

Aim To develop an HPLC method for determination of the content of Baicalin in Julan Granules.Methods A Waters:Symmetry C18 column(3.9mm×150mm,5μm) was used served as the chromatographic column.The mobile phase consisted of methanol-water-phosphate acid(47∶53∶0.2).The UV detection wavelength was 280nm.The flow rate was 1.0ml/min.Results The standard curve was linear in the range of 0.08~0.72μg,r=0.999 6.The average recovery was 98.76%,RSD=1.52%(n=9).Conlusion The method is simple,effective and accurate, and can be used for quality control of Julan Granules.

Key concepts: Baicalin, Chromatography, High-performance liquid chromatography, Chemistry, Content determination, Linear range, Recovery rate, Detection limit

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