2007•Zhongguo shiyan fangjixue zazhiRequires access

Determination of Ferulic Acid in Bitong Yaojiu by HPLC

Cuiying Zhang, LI Zhen-guo

Abstract

Objective:To develop and HPLC method for determination of ferulic acid in Bitong Yaojiu.Methods:Analysis was carried out on a C_(18)(150 mm×4.6 mm,5 μm)column eluled with,acetonitrile-0.1% phosphoric acid buffer(20∶80)as the mobile phase with UV detection at 316 nm.Results:The linear relationship of ferulic acid was in the range of 4.072～101.8 μg/mL,the recovery was 99.31%(RSD 3.66%,n=6).Conclusion:This method is simple,accurate and reproducible.

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Key concepts: Ferulic acid, Phosphoric acid, Chromatography, High-performance liquid chromatography, Chemistry, Acetonitrile, Linear range, Linear relationship