ScholarLens Back to search
2013•Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access

Determination of Ferulic Acid in Huoxuezhitong Solution by HPLC

Shen Ru

Open publisher page 0 citations

Abstract

Objective To establish an HPLC quantitative method for the determination of ferulic acid in Huoxuezhitong Solution. Methods An HPLC method was used for determining the content of ferulic acid. The chromatography column was Inertsil ODS-SP C 18(250 mm × 4. 6 mm,5 μm) and the column temperature was set at 30 ℃. The mobile phase was acetonitrile-0. 05% phosphoric acid solution(17∶ 83) at a flow rate of 1. 0 ml·min-1. The detection wavelength was 320 nm. Results The calibration curve of ferulic acid showed a good linear relationship over the range of 1. 77-28. 26 μg·ml1(r =0. 9998). The average recovery of ferulic acid was 100. 22% and RSD was 1. 67%(n = 9). Conclusion This method is simple,accurate,and reproducible. It can be used for the determination of ferulic acid in Huoxuezhitong solution.

About this research paper

What this paper is about

Objective To establish an HPLC quantitative method for the determination of ferulic acid in Huoxuezhitong Solution. Methods An HPLC method was used for determining the content of ferulic acid. The chromatography column was Inertsil ODS-SP C 18(250 mm × 4. 6 mm,5 μm) and the column temperature was set at 30 ℃. The mobile phase was acetonitrile-0. 05% phosphoric acid solution(17∶ 83) at a flow rate of 1. 0 ml·min-1. The detection wavelength was 320 nm. Results The calibration curve of ferulic acid showed a good linear relationship over the range of 1. 77-28. 26 μg·ml1(r =0. 9998). The average recovery of ferulic acid was 100. 22% and RSD was 1. 67%(n = 9). Conclusion This method is simple,accurate,and reproducible. It can be used for the determination of ferulic acid in Huoxuezhitong solution.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To establish an HPLC quantitative method for the determination of ferulic acid in Huoxuezhitong Solution. Methods An HPLC method was used for determining the content of ferulic acid. The chromatography column was Inertsil ODS-SP C 18(250 mm × 4. 6 mm,5 μm) and the column temperature was set at 30 ℃. The mobile phase was acetonitrile-0. 05% phosphoric acid solution(17∶ 83) at a flow rate of 1. 0 ml·min-1. The detection wavelength was 320 nm. Results The calibration curve of ferulic acid showed a good linear relationship over the range of 1. 77-28. 26 μg·ml1(r =0. 9998). The average recovery of ferulic acid was 100. 22% and RSD was 1. 67%(n = 9). Conclusion This method is simple,accurate,and reproducible. It can be used for the determination of ferulic acid in Huoxuezhitong solution.

Key concepts: Ferulic acid, Phosphoric acid, Chromatography, High-performance liquid chromatography, Calibration curve, Chemistry, Acetonitrile, Content determination

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of Ferulic Acid in Huoxuezhitong Solution by HPLC — Research Paper | ScholarLens