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2011•Journal of Hubei University of Chinese MedicineRequires access

Determination of Ferulic acid in Toutongning Mixture by HPLC

Jinjin Cui

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Abstract

Objective To develop a method for the determination of Ferulic acid in Toutongning mixture.Method HPLC method was established.The Chromatographic column was C18.The mobile phase was acetonitrile-0.085% phosphoric acid solution(17∶83).The detection wavelength was 316 nm and the flow rate was l mL/min.Results The good linearity was obtained over the range of 9.8 μg/mL-98 μg/mL for ferulic acid.The average recovery was 101.0%,RSD=1.42%(n=6).Conclusion The method can be used for quantitative determination of this preparation.

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What this paper is about

Objective To develop a method for the determination of Ferulic acid in Toutongning mixture.Method HPLC method was established.The Chromatographic column was C18.The mobile phase was acetonitrile-0.085% phosphoric acid solution(17∶83).The detection wavelength was 316 nm and the flow rate was l mL/min.Results The good linearity was obtained over the range of 9.8 μg/mL-98 μg/mL for ferulic acid.The average recovery was 101.0%,RSD=1.42%(n=6).Conclusion The method can be used for quantitative determination of this preparation.

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Available abstract

Objective To develop a method for the determination of Ferulic acid in Toutongning mixture.Method HPLC method was established.The Chromatographic column was C18.The mobile phase was acetonitrile-0.085% phosphoric acid solution(17∶83).The detection wavelength was 316 nm and the flow rate was l mL/min.Results The good linearity was obtained over the range of 9.8 μg/mL-98 μg/mL for ferulic acid.The average recovery was 101.0%,RSD=1.42%(n=6).Conclusion The method can be used for quantitative determination of this preparation.

Key concepts: Ferulic acid, Chromatography, Phosphoric acid, High-performance liquid chromatography, Chemistry, Acetonitrile, Chromatographic separation, Organic chemistry

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