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2002•Journal of Analytical ScienceRequires access

Determination of Sterol by HPLC with Evaporative Light-scatter Detector

Feng Liu

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Abstract

An HPLC ELSD method for the determination of sterol has been described. Chromatographic analysis was carried out on a Supecol Discovery C 18 column (250×4.6 mm i.d.) with methanol as the mobile phase. The average rocoveries were from 98.5% to 101%(n=5), and the relative standard deriation (RSD) was below 0.45%. The method was simple, rapid and accurate.

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What this paper is about

An HPLC ELSD method for the determination of sterol has been described. Chromatographic analysis was carried out on a Supecol Discovery C 18 column (250×4.6 mm i.d.) with methanol as the mobile phase. The average rocoveries were from 98.5% to 101%(n=5), and the relative standard deriation (RSD) was below 0.45%. The method was simple, rapid and accurate.

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Available abstract

An HPLC ELSD method for the determination of sterol has been described. Chromatographic analysis was carried out on a Supecol Discovery C 18 column (250×4.6 mm i.d.) with methanol as the mobile phase. The average rocoveries were from 98.5% to 101%(n=5), and the relative standard deriation (RSD) was below 0.45%. The method was simple, rapid and accurate.

Key concepts: Chemistry, Chromatography detector, Chromatography, Sterol, High-performance liquid chromatography, Detector, Methanol, Analytical Chemistry (journal)

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