2009•Science and Technology of Food IndustryRequires access

Quantification analysis of glycoglycerolipids by reversd-phase high performance liquid chromatography(RP-HPLC) with evaporative light scattering detection(ELSD)

Zhiguo Jiang

Abstract

A method for the determination of glycoglycerolipid QGMG(18:3) 、TGMG(18:3) 、QGMG(18:2) and TGMG(18:2) by reversd-phase high performance liquid chromatography (RP-HPLC) with evaporative light scattering detection(ELSD) was developed.Glycoglycerolipids were successfully separated on Waters Symmetry C 18 (4.6mm×150mm,5μm),using acetonitrile-water(60:40,v/v) as mobile phase at a flow rate 1.0mL/min.The drift tube temperature and air carrier gas pressure of ELSD were 60℃ and 0.35MPa,respectively.The linear ranges of the four components were 0.05～0.5mg/mL.The spiked recoveries from pumpkin samples were 96.7%～101.3%.It was proved that the method with HPLC-ELSD was reliable,simple,well separated and precise,that can be used as principles of quantitative analysis.

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Key concepts: Chromatography detector, Chromatography, High-performance liquid chromatography, Chemistry, Drift tube, Analytical Chemistry (journal), Acetonitrile, Volumetric flow rate