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Content Determination of Germacrone in Rhizoma Curcuma by HPLC

Xie Yongzhong

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Abstract

OBJECTIVE:To determine the content of germacrone in rhizoma curcuma by HPLC. METHODS: C18 was the chromatographic column, the mobile phase was acetonitrile and water(gradient elution) with a flow rate of 1.0ml/min, the detection wavelength was at 214nm. RESULTS:The linear range of germacrone was 0.95～285μg/ml (r=0.9 999),the mean recovery rate was 97.89%(RSD=2.52%,n=5).CONCLUSION:The method is simple and reliable and which can be used for the content determination and the quality control of rhizoma curcuma.

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What this paper is about

OBJECTIVE:To determine the content of germacrone in rhizoma curcuma by HPLC. METHODS: C18 was the chromatographic column, the mobile phase was acetonitrile and water(gradient elution) with a flow rate of 1.0ml/min, the detection wavelength was at 214nm. RESULTS:The linear range of germacrone was 0.95～285μg/ml (r=0.9 999),the mean recovery rate was 97.89%(RSD=2.52%,n=5).CONCLUSION:The method is simple and reliable and which can be used for the content determination and the quality control of rhizoma curcuma.

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Available abstract

OBJECTIVE:To determine the content of germacrone in rhizoma curcuma by HPLC. METHODS: C18 was the chromatographic column, the mobile phase was acetonitrile and water(gradient elution) with a flow rate of 1.0ml/min, the detection wavelength was at 214nm. RESULTS:The linear range of germacrone was 0.95～285μg/ml (r=0.9 999),the mean recovery rate was 97.89%(RSD=2.52%,n=5).CONCLUSION:The method is simple and reliable and which can be used for the content determination and the quality control of rhizoma curcuma.

Key concepts: Curcuma, Chromatography, Chemistry, Gradient elution, High-performance liquid chromatography, Linear range, Content determination, Traditional medicine

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