Determination of Content of Germacrone in Curcuma kwangsiensis by RP-HPLC

Objective:To establish a HPLC method for the determination of germacrone in Curcuma kwangsiensis.Method:After using the orthogonal test to optimize extracting conditions,germacrone was separated on Phenomenex C18(4.6 mm×250 mm,5 μm) column and detected at 217 nm,with 76% acetonitrile as mobile phase,and the flow rate was 1.0 mL·min-1.Result:Germacrone was linear in the range of 0.056 7-2.324 7 μg(r=0.999 99) and the average recovery of germacrone was 98.05% with RSD 0.69%.The most and the least content of germacrone in C.kwangsiensis was from Rong county and Pingnan county respectively.The germacrone in processed root tubers and stems and leaves of C.kwangsiensis were much less than that in the root tubers dried in the shade.Conclusion:The method is simple,accurate and rapid.The conternt of germacrone in C.kwangsiensis from different producing areas,medicinal parts and processed products make a great difference.