In vitro culture of young embryo of sweet and waxy maize

Young embryos of four sweet and waxy maize inbred lines(N1,N28,T5,T6)were cultured in vitro,and factors influecing embryogenic callus induction,subculture,differentiation and plant regeneration were analyzed.The results indicated that callus could be induced from young embryos of all the four lines tested.The induction rates differed with different genotypes and media.Young embryo of 1～2 mm in size was most suitable for callus induction 2,4-D was the necessary phytohormone for callus induction and subculture,and the suitable concentration was 2.0 mg/L.AgNO3 was propitious to induction and growth of embryogenic callus,the optimum concentration was 10 mg/L.On the medium containing AgNO3,the induction rate of embryogenic callus was 27.15% higher than that of the control.