Optimization of Induction Conditions of Immature Embryo Callus for Four Maize Inbred Lines

Ten culture media with different plant hormones for callus induction were designed,in order to analyze the callus induction conditions of four excellent maize inbred lines.The results showed that genotypes had significant effects on callus induction,and there were interaction between genotype and medium;2,4-dichlorophenoxyacetic acid(2,4-D) was necessary for callus induction,and the optimal concentration for embryogenic callus formation was 2.0-3.0mg/L;The length of embryo between 1.5-2.0mm was the best time,when higher induction frequency and better quality of embryo callus were gotten;The addition of 10mg/L AgNO3 to the N6 medium could obviously stimulated embryo callus formation,and the addition of 6-BA had no significant effect on induction frequency.