2005•Xi'nan nongye xuebaoRequires access
Analysis of the factors affecting the production of maize embryogenic callus by immature embryo.
Cao Mo-ju, Ying Zhang, Yuzhen Liu, Sun Qing-quan, Rong Ting-zhao
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Abstract
5 maize inbred lines were studied for the induction percentage of immature embryogeneic callus in this experiment.Different geneotypes and different induction mediums were evaluated.The relationship between induction percentage and subculture percentage was analyzed.The results show :there are significant differences among the different induction percentage of 5 maize inbred lines on the same conditions.The content of 2,4-D in the induction mediums have an evident effect on the callus induction percentage of immature embryo.The accession of NAA to the induction mediums can not increase the induction percentage of embryogenic callus evidently.The correlation between embryogenic callus induction percentage and subculture percentage did not reach to significant level at this experiment,this indicates that embryogenic callus induction percentage and embryogenic callus subculture percentage may be controlled by different genetic factors.