Effect of mAKAPβ on AngII-induced cardiac hypertrophy and its mechanism

Objective To investigate the effect of mAKAPβ on Angll-induced cardiac hypertrophy and its mechanism.Methods Cultured cardiomyocytes from neonatal rats were treated with Angll,the morphology of cardiomyocytes were observed and the expression of mAKAPβ was measured.The mAKAPβ-shRNA was constructed for RNA interference,the expression of mAKAPβ in Angll-treated rat cardiomyocytes was detected after RNA interference.The changes of ERK in cardiomyocytes were assessed before and after Angll stimulation.Results In Angll treated rat cardiac myocytes the areas of cells were larger than those of untreated cardiomyocytes,and the expression of hypertrophy marker ANP was higher in Angll treated cells;however the expression level of mAKAPβ remained unchanged.RNA interference inhibited the expression of mAKAPβ protein,and the hypertrophy of cardiomyocyte induced by Angll was attenuated.Conclusion Angll can induce hypertrophy in rat cardiac myocytes,in which mAKAPβ protein is involved.