Age-related changes of muscle A-kinase anchoring protein β in mouse myocytes and its relation with hypertrophy of cardiac ventricular myocytes

Objective To investigate the age-related changes of muscle A-kinase anchoring protein β (mAKAPβ) in mouse myocytes and its relation with hypertrophy of cardiac myocytes. Methods The cardiac myocyte was extracted from mice on day 1,day 7,day 40 and day 90 after birth. Expression of mAKAPβmRNA was examined by real-time PCR in mouse cardiac myocytes before and after treatment with leukocyte inhibition factor (LIF). Results Expression of mAKAPβ increased with age in mouse myocytes; and the expression levels on day 1,day 7,day 40 and day 90 after birth were 1.00±0.00,1.52±0.06,1.81±0.14 and 2.20±0.18,respectively (P0.01 or 0.05). After LIF treatment for 48 hours,the cardiac myocytes became hypertrophy compared to untreated group (area of myocyte: [3 107.84±731.98] vs. [2,348.19±533.00],P0.05). Meanwhile,mAKAPβ expression in the LIF treatment group was significantly higher than that in the control group ([1.00±0.00] vs. [1.62±0.02],P0.01). Conclusion mAKAPβ expression in mouse ventricular myoctye increases with age,and the expression of mAKAPβ is obviously higher in hypertrophic cardiac ventricular myoctyes compared with the normal ones.