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2010•Tianjin Agricultural SciencesRequires access

Study on the Factory Proliferation of Anoectochilus Formosanu

HU Song-mei

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Abstract

Using tender stem as explant, tissue culture and factory proliferation of Anoectochilus formosanu had been studied. The results indicated that the optimum medium for multiplication of cluster buds was MS + 6-BA 0.5 mg·L-1 + NAA 0.2 mg·L-1;and the medium for rooting was 1/2MS + 6-BA 0.1 mg·L-1+NAA 0.5 mg·L-1. The transplanting of young plants produced from in-vitro culture has also been studied. The results showed that the optimum culture medium is 2 Humus + 1 vermiculite and Spray 1/4 MS once every 5 d, Spray 1 000 times the amino acid once every 15 d.

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What this paper is about

Using tender stem as explant, tissue culture and factory proliferation of Anoectochilus formosanu had been studied. The results indicated that the optimum medium for multiplication of cluster buds was MS + 6-BA 0.5 mg·L-1 + NAA 0.2 mg·L-1;and the medium for rooting was 1/2MS + 6-BA 0.1 mg·L-1+NAA 0.5 mg·L-1. The transplanting of young plants produced from in-vitro culture has also been studied. The results showed that the optimum culture medium is 2 Humus + 1 vermiculite and Spray 1/4 MS once every 5 d, Spray 1 000 times the amino acid once every 15 d.

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Available abstract

Using tender stem as explant, tissue culture and factory proliferation of Anoectochilus formosanu had been studied. The results indicated that the optimum medium for multiplication of cluster buds was MS + 6-BA 0.5 mg·L-1 + NAA 0.2 mg·L-1;and the medium for rooting was 1/2MS + 6-BA 0.1 mg·L-1+NAA 0.5 mg·L-1. The transplanting of young plants produced from in-vitro culture has also been studied. The results showed that the optimum culture medium is 2 Humus + 1 vermiculite and Spray 1/4 MS once every 5 d, Spray 1 000 times the amino acid once every 15 d.

Key concepts: Explant culture, Vermiculite, Transplanting, Tissue culture, Humus, Horticulture, Botany, In vitro

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