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2006•Journal of Wenshan Teachers' CollegeRequires access

A Study on Embryo Germination and Proliferation in Vitro of Anoectochilus Chapaesis

Ang Tie

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Abstract

This paper presented the optimal culture conditions for the in vitro embryo germination and proliferation in vitro of Anoectochilus chapaesis.The results were as follows: the seeds germinated well in Kyoto medium without growth regulators,and new shoots were differentiated from protocorm when 10% CM were added.In a second experiment,the stems were then used as explants and produced new shoots best in the medium of Hyponex l +6-BA 1 mg/L+NAA (0.01)/L;the best proliferation medium was Hyponex l + BA 0.5 mg/L+NAA 0.1 mg/L;and the best rooting medium was Hyponex l+6-BA 0.05mg/L+NAA0.1 mg/L.

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What this paper is about

This paper presented the optimal culture conditions for the in vitro embryo germination and proliferation in vitro of Anoectochilus chapaesis.The results were as follows: the seeds germinated well in Kyoto medium without growth regulators,and new shoots were differentiated from protocorm when 10% CM were added.In a second experiment,the stems were then used as explants and produced new shoots best in the medium of Hyponex l +6-BA 1 mg/L+NAA (0.01)/L;the best proliferation medium was Hyponex l + BA 0.5 mg/L+NAA 0.1 mg/L;and the best rooting medium was Hyponex l+6-BA 0.05mg/L+NAA0.1 mg/L.

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Available abstract

This paper presented the optimal culture conditions for the in vitro embryo germination and proliferation in vitro of Anoectochilus chapaesis.The results were as follows: the seeds germinated well in Kyoto medium without growth regulators,and new shoots were differentiated from protocorm when 10% CM were added.In a second experiment,the stems were then used as explants and produced new shoots best in the medium of Hyponex l +6-BA 1 mg/L+NAA (0.01)/L;the best proliferation medium was Hyponex l + BA 0.5 mg/L+NAA 0.1 mg/L;and the best rooting medium was Hyponex l+6-BA 0.05mg/L+NAA0.1 mg/L.

Key concepts: Germination, Explant culture, Shoot, In vitro, Embryo, Botany, Biology, Liquid culture

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