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2007•Journal of Huizhou UniversityRequires access

Study on Tissue Culture and Transplanting Techniques of Anoectochilus Roburghii

Zhaogui Chen

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Abstract

The study was aimed at obtaining optimized culture media for buds induction and multiplication,and root induction of anoec- tochilus roburghii to set up the Tissue Culture technolygy of anoectochilus roburghii.The result showed that MS plus(NAA0.5 mg/L+ 6-BA3.0 mg/+ KT1.0 mg/L)is the best media for buds induction.MS adding(NAA0.5 mg/L+6-BA3.0 mg/L)is the best media for multiplication.1/2 MS plus(IBA1.0 mg/L+NAA1.0 mg/L+0.5% activated carbon+casein hydroly-sate is the best media for root induction.Used common soil and organic manure soil with sand(1:1:1)mixed,tbe surviving rate would be 90.9%.

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What this paper is about

The study was aimed at obtaining optimized culture media for buds induction and multiplication,and root induction of anoec- tochilus roburghii to set up the Tissue Culture technolygy of anoectochilus roburghii.The result showed that MS plus(NAA0.5 mg/L+ 6-BA3.0 mg/+ KT1.0 mg/L)is the best media for buds induction.MS adding(NAA0.5 mg/L+6-BA3.0 mg/L)is the best media for multiplication.1/2 MS plus(IBA1.0 mg/L+NAA1.0 mg/L+0.5% activated carbon+casein hydroly-sate is the best media for root induction.Used common soil and organic manure soil with sand(1:1:1)mixed,tbe surviving rate would be 90.9%.

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Available abstract

The study was aimed at obtaining optimized culture media for buds induction and multiplication,and root induction of anoec- tochilus roburghii to set up the Tissue Culture technolygy of anoectochilus roburghii.The result showed that MS plus(NAA0.5 mg/L+ 6-BA3.0 mg/+ KT1.0 mg/L)is the best media for buds induction.MS adding(NAA0.5 mg/L+6-BA3.0 mg/L)is the best media for multiplication.1/2 MS plus(IBA1.0 mg/L+NAA1.0 mg/L+0.5% activated carbon+casein hydroly-sate is the best media for root induction.Used common soil and organic manure soil with sand(1:1:1)mixed,tbe surviving rate would be 90.9%.

Key concepts: Transplanting, Tissue culture, Manure, Multiplication (music), Chemistry, Horticulture, Botany, Biology

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