Influence of Bushen Huatan Decoction on TNF-α and IL-6 in Ovariectomized Rats

Objective: To observe the influence of Bushen Huatan decoction on tumor necrosis factor( TNF-α) and interleukin-6( IL-6) in ovariectomized rats and to explore the therapeutic mechanism of the drugs.Method: Female SD rats were divided into five groups: model group( A),sham operation group( B),livial group( C),Bushen Huatan decoction low dose group( D) and high dose group( E). Except for group B,all rats were ovariectomized. Drug treatment began at three months after the operation. Group C was treated with livial at the dose 0. 23 mg·kg-1,group D and E were treated with the low dose 9.4 g·kg-1the large dose 18. 8 g·kg-1and Bushen Huatan decoction respectively A,B group recewed the same volume of physiological saline. After 8weeks intragastric administration by once a day,the rat's bone mineral density was measured by dual energy X-ray absorptionmetry. Levels of TNF-α and IL-6 in bone tissue were determined by using immunohistoehemistry techniques. Result: Bushen Huatan decoction can evidently increase bone density as well as the trabecular bone volume and density,and decrease the levels of TNF-α and IL-6 in bone. Conclusion: Bushen Huatan decoction can effectly prevent osteoporosis in female rats induced by ovariectomy,and the mechanism maybe lowing levels of TNF-α and IL-6 in bone tissue.