2008•The Journal of Pharmaceutical PracticeRequires access

Determination of baicalin in Xiaoer Runfei Oral liquid by HPLC

Tan Sheng-jian

Abstract

Objective:To develop an HPLC quantitative method for the determination of baicalin in Xiaoer Runfei Oral liquid.Methods:HPLC system consisted an Agilent C_(18)column(ZORBAX Eclipse XDB-C18,4.6 mm×150 mm,5μm)and a mixture of methanol-water-phosphoric acid(38:62:0.5)as mobile phase.The flow rate was 1.0 mL/min and was monitored at 278 nm. Results:The retention time of baicalin was 20.6 min.The regress equation for baicalin was Y=0.060 47X+1.131,r=0.999 9,and the linear range was 62.84～314.2μg/mL.The average recovery of baicalin was(n=6)103.3%,RSD 0.43%.Conclusion:The method was sensitive,time-saving and accurate.

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Key concepts: Baicalin, High-performance liquid chromatography, Chromatography, Phosphoric acid, Chemistry, Linear relationship, Linear range, Methanol