Determination on baicalin in Xiaoer Tuire oral liquid by HPLC
Yeqing Chen
Abstract
Yeqing Chen
Abstract
Objective:to establish HPLC method for the determination of baicalin in the Xiaoer Tuire oral liquid. Method: the HPLC method was carried on C 18 column using method-water-codeine(45∶55∶0.2) as mobile phase. Detection wavelengthwas set at 315nm. Results: in the HPLC method the calibration curve for baicalin was linear over the range of 0.15~1.2 μg/mL. The average recovery was 98.07% with RSD=1.05%. Conclusions: the method is simple, convenient and reliablefor quality control of the liquids.
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Objective:to establish HPLC method for the determination of baicalin in the Xiaoer Tuire oral liquid. Method: the HPLC method was carried on C 18 column using method-water-codeine(45∶55∶0.2) as mobile phase. Detection wavelengthwas set at 315nm. Results: in the HPLC method the calibration curve for baicalin was linear over the range of 0.15~1.2 μg/mL. The average recovery was 98.07% with RSD=1.05%. Conclusions: the method is simple, convenient and reliablefor quality control of the liquids.
Key concepts: Baicalin, Chromatography, High-performance liquid chromatography, Calibration curve, Chemistry, Linear range, Detection limit