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Determination of Astragaloside IV in Yiqiyangxue Oral Liquid by HPLC-ELSD

Zijing Wu

Abstract

[Objective] To establish a method for determination of astragaloside Ⅳ in Yiqiyangxue oral liquid.[Method] Ultrasonic extraction and HPLC–ELSD.Mobile phase consisted of acetonitrile–water(35 ∶ 65).[Results] The content of astragaloside Ⅳ ashowed good linear relationship in the range of 4.06-8.12 μg,and the average recovery of astragaloside Ⅳ was 96.44% with 1.24% relative standard deviation.[Conclusion] The method is simple and accurate,also applicable for the determination and quality control of Yiqiyangxue oral liquid.

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Key concepts: Astragaloside, Chromatography, High-performance liquid chromatography, Chromatography detector, Relative standard deviation, Extraction (chemistry), Linear range, Chemistry