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Quantitative Comparison of Puerarin in Two Puerariae Extracts

XU Hai-bo

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Abstract

The content determination of puerarin of the two Puerariae extracts has been carried out by HPLC with C 18 chemical bonded silica gel as a solid phase, Methanol-Water(25∶75) as a mobile phase, and UV detection wavelength at 250 nm. The average recovery of the added index was 98.37% (n=6);RSD was 0.54%. There was a good linear relationship between the concentration of puerarin and absorption area value in range of 0.16～0.80 μg(γ=0.999 8). The determination of puerarin in extracts of Chaige 〔Pueraria lobata (Willd.) Ohwi〕is higher than it in extracts of Fenge (Pueraria thonsonii Benth.).

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The content determination of puerarin of the two Puerariae extracts has been carried out by HPLC with C 18 chemical bonded silica gel as a solid phase, Methanol-Water(25∶75) as a mobile phase, and UV detection wavelength at 250 nm. The average recovery of the added index was 98.37% (n=6);RSD was 0.54%. There was a good linear relationship between the concentration of puerarin and absorption area value in range of 0.16～0.80 μg(γ=0.999 8). The determination of puerarin in extracts of Chaige 〔Pueraria lobata (Willd.) Ohwi〕is higher than it in extracts of Fenge (Pueraria thonsonii Benth.).

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Available abstract

The content determination of puerarin of the two Puerariae extracts has been carried out by HPLC with C 18 chemical bonded silica gel as a solid phase, Methanol-Water(25∶75) as a mobile phase, and UV detection wavelength at 250 nm. The average recovery of the added index was 98.37% (n=6);RSD was 0.54%. There was a good linear relationship between the concentration of puerarin and absorption area value in range of 0.16～0.80 μg(γ=0.999 8). The determination of puerarin in extracts of Chaige 〔Pueraria lobata (Willd.) Ohwi〕is higher than it in extracts of Fenge (Pueraria thonsonii Benth.).

Key concepts: Puerarin, Pueraria, Chromatography, Chemistry, High-performance liquid chromatography, Radix (gastropod), Traditional medicine, Botany

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