High performance liquid chromatographic determination of Tuihuang oral liquid
Yang Yi
Abstract
Yang Yi
Abstract
OBJECTIVE To estimate an HPLC for determination of gardenoside in Tuihuang oral liquid.METHODS Chromatographic olumn C 18 ODS;Mobile phase MeOH water (20∶80);detection wavelength 240 nm.RESULTS The linear regression equation was Y =1566700 x -4691, r =0.9998.The average recovery was 99.25% with RSD =2.78%( n =5).CONCLUSION This is a simple,accurate method which can be used for quantitative analysis of Tuihuang oral liquid.
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OBJECTIVE To estimate an HPLC for determination of gardenoside in Tuihuang oral liquid.METHODS Chromatographic olumn C 18 ODS;Mobile phase MeOH water (20∶80);detection wavelength 240 nm.RESULTS The linear regression equation was Y =1566700 x -4691, r =0.9998.The average recovery was 99.25% with RSD =2.78%( n =5).CONCLUSION This is a simple,accurate method which can be used for quantitative analysis of Tuihuang oral liquid.
Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Liquid liquid, Linear regression, Linear relationship, Phase (matter), Liquid phase