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Determination of the contents of Paeonol in Cortex Muotan and Qijudihuang Pills by RP-HPLC

Hua Zhang

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Abstract

Objective To establish a method for the quantitative determination of the contents of Paeonol in Cortex Muotan and Qijudihuang Pills.Methods Paeonol were separated and determined by reversed phase high performance liquid chromatography(RP-HPLC),with a CLO-ODS column(150×(4.6 mm)) and a mixture of methanol-phosphate buffer(pH3.1)(42∶58)as mobile phase(particle size 5 microns).The flow rate was 1 ml/min.The column temperature was 30℃.The detection wavelength was 299 nm.The retention time for Paeonol was 7.5 min.Results Paeonol in Cortex Muotan has a good linearity,and the average recovery with RSD(n=6)is 97.86%±0.64% for paeonol.The linear calibration curve for Paeonol was obtained in the range 5.0~50.0 micrograms with the correlation coefficient 0.9997.Conclusion The method is simple,sensitive,rapid and accurate.

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Objective To establish a method for the quantitative determination of the contents of Paeonol in Cortex Muotan and Qijudihuang Pills.Methods Paeonol were separated and determined by reversed phase high performance liquid chromatography(RP-HPLC),with a CLO-ODS column(150×(4.6 mm)) and a mixture of methanol-phosphate buffer(pH3.1)(42∶58)as mobile phase(particle size 5 microns).The flow rate was 1 ml/min.The column temperature was 30℃.The detection wavelength was 299 nm.The retention time for Paeonol was 7.5 min.Results Paeonol in Cortex Muotan has a good linearity,and the average recovery with RSD(n=6)is 97.86%±0.64% for paeonol.The linear calibration curve for Paeonol was obtained in the range 5.0~50.0 micrograms with the correlation coefficient 0.9997.Conclusion The method is simple,sensitive,rapid and accurate.

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Available abstract

Objective To establish a method for the quantitative determination of the contents of Paeonol in Cortex Muotan and Qijudihuang Pills.Methods Paeonol were separated and determined by reversed phase high performance liquid chromatography(RP-HPLC),with a CLO-ODS column(150×(4.6 mm)) and a mixture of methanol-phosphate buffer(pH3.1)(42∶58)as mobile phase(particle size 5 microns).The flow rate was 1 ml/min.The column temperature was 30℃.The detection wavelength was 299 nm.The retention time for Paeonol was 7.5 min.Results Paeonol in Cortex Muotan has a good linearity,and the average recovery with RSD(n=6)is 97.86%±0.64% for paeonol.The linear calibration curve for Paeonol was obtained in the range 5.0~50.0 micrograms with the correlation coefficient 0.9997.Conclusion The method is simple,sensitive,rapid and accurate.

Key concepts: Paeonol, Chromatography, High-performance liquid chromatography, Chemistry, Calibration curve, Correlation coefficient, Phosphate buffered saline, Content determination

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