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Determination of paeonol in the bulk drug and injection by RP-HPLC

Sun Yan

Open publisher page 1 citations

Abstract

AIM To determine the concentration of paeonol in the bulk drug and injection, a method of reversed-phase high performance liquid chromatography (RP-HPLC) was developed. METHODS Lichrospher C_ 18 column(4.6 mm×250 mm, 5 μm)was used with methanol-acetonitrile-water(30∶40∶30)as mobile phase at a flow rate of 0.8 ml·min -1 . 20 μl of sample was injected into the C_ 18 column where the temperature was 25℃. The detection wavelength was 274 nm. RESULTS The linear regression equation for paeonol was = 3 772.525 8 X- 0.747 4 (r = 1.000 0, n =5) under the linearity range from 0.02 mg·L -1 to 25.60 mg·L -1 . The average recovery was 99.87%. The relative standard deviations of the intra-day and inter-day were less than 4%. CONCLUSION The method is simple and rapid, which may be used for the determination of paeonol and its preparation for different purpose.

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AIM To determine the concentration of paeonol in the bulk drug and injection, a method of reversed-phase high performance liquid chromatography (RP-HPLC) was developed. METHODS Lichrospher C_ 18 column(4.6 mm×250 mm, 5 μm)was used with methanol-acetonitrile-water(30∶40∶30)as mobile phase at a flow rate of 0.8 ml·min -1 . 20 μl of sample was injected into the C_ 18 column where the temperature was 25℃. The detection wavelength was 274 nm. RESULTS The linear regression equation for paeonol was = 3 772.525 8 X- 0.747 4 (r = 1.000 0, n =5) under the linearity range from 0.02 mg·L -1 to 25.60 mg·L -1 . The average recovery was 99.87%. The relative standard deviations of the intra-day and inter-day were less than 4%. CONCLUSION The method is simple and rapid, which may be used for the determination of paeonol and its preparation for different purpose.

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Available abstract

AIM To determine the concentration of paeonol in the bulk drug and injection, a method of reversed-phase high performance liquid chromatography (RP-HPLC) was developed. METHODS Lichrospher C_ 18 column(4.6 mm×250 mm, 5 μm)was used with methanol-acetonitrile-water(30∶40∶30)as mobile phase at a flow rate of 0.8 ml·min -1 . 20 μl of sample was injected into the C_ 18 column where the temperature was 25℃. The detection wavelength was 274 nm. RESULTS The linear regression equation for paeonol was = 3 772.525 8 X- 0.747 4 (r = 1.000 0, n =5) under the linearity range from 0.02 mg·L -1 to 25.60 mg·L -1 . The average recovery was 99.87%. The relative standard deviations of the intra-day and inter-day were less than 4%. CONCLUSION The method is simple and rapid, which may be used for the determination of paeonol and its preparation for different purpose.

Key concepts: Paeonol, Chromatography, High-performance liquid chromatography, Chemistry, Acetonitrile, Methanol, Relative standard deviation, Analytical Chemistry (journal)

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