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Determination of Paeonol in Banlonggushen Capsule by HPLC

Zhang Qiu-hai

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Abstract

Objective To determine the content of paeonol in Banlonggushen Capsule. Method Sino Chirom C18 column, mobile phase was methanol-0.25% acetic acid (45 ∶ 55). The detection wavelength was at 274 nm and the flow rate was 1.0 mL/min. Result The linearity was obtained in the range of 0.0532~0.3192 μg (r =0.9999). The average recovery was 100.2%. Conclusion The method was simple and reliable. It is suitable for the determination of paeonol in Banlonggushen Capsule.

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What this paper is about

Objective To determine the content of paeonol in Banlonggushen Capsule. Method Sino Chirom C18 column, mobile phase was methanol-0.25% acetic acid (45 ∶ 55). The detection wavelength was at 274 nm and the flow rate was 1.0 mL/min. Result The linearity was obtained in the range of 0.0532~0.3192 μg (r =0.9999). The average recovery was 100.2%. Conclusion The method was simple and reliable. It is suitable for the determination of paeonol in Banlonggushen Capsule.

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Available abstract

Objective To determine the content of paeonol in Banlonggushen Capsule. Method Sino Chirom C18 column, mobile phase was methanol-0.25% acetic acid (45 ∶ 55). The detection wavelength was at 274 nm and the flow rate was 1.0 mL/min. Result The linearity was obtained in the range of 0.0532~0.3192 μg (r =0.9999). The average recovery was 100.2%. Conclusion The method was simple and reliable. It is suitable for the determination of paeonol in Banlonggushen Capsule.

Key concepts: Paeonol, Capsule, Chromatography, High-performance liquid chromatography, Chemistry, Medicine, Biology, Botany

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