Cloning and sequence analysis of partial fragment of prolactin receptor gene in yaks

Partial conversed sequence of prolactin receptor(PRLR) gene of yaks is obtained by PCR in order to provide information for studying the expression of PRLR gene in yak mammary tissue.The total mRNA of yak mammary tissue is isolated,and RT-PCR based on the specific primer,which is designed with Software primer 5 according to cow PRLR gene of NCBI.The result shows the sequence is 577 bp.The 577 bp DNA is ligated with Plasmid PMD18-simple,and then transformed into bacillus coli.The positive clones which are identified by PCR(transformed bacillus coli as template) are used for sequencing and deducing amino acid sequence.Comparing the obtained sequence and sequence of amino acids with those in buffalo,cow,sheep,human,rat and mouse respectively,99.13%,97.40%,93.41%,71.07%,60.20% in nucleotide homology and 100%,97.40%,95.83%,82.38%,72.22% in the amino acid homology are observed.