Resistance Analysis of Extended-spectrum β-Lactamases (ESBLs)-producing Bacteria

OBJECTIVE To investigate the characteristics about drug resistance of extended-spectrum β-lactamases(ESBLs)-producing bacteria for the guidance of clinical rational use of antibiotics.METHODS To detect ESBLs by ATB microbiological system.We used Kirby-Bauer(K-B)test for antibiotics susceptibility test.The results were evaluated based on National Committee for Clinical Laboratory Standards(NCCLS) of the USA in 2005.The data were analyzed by using WHONET-5 software.RESULTS Among the isolated 431 strains of ESBLs-producing bacteria(42.05%),330 strains were of Escherichia coli(49.03%),and 101 strains of Klebsiella pneumoniae(28.69%);the resistant rate of ESBLs-producing bacteria to carbapenem(meropemem,imipenem-cilastatin),cefoxitin,complex antibiotics containing β-lactamases inhibitor,and amikacin was relatively less(0-28.4%),the resistant rate to other antimicrobial drugs was higher.CONCLUSIONS It is very important to determine ESBLs-producing bacteria.Correctly selecting antimicrobial drugs for infection treatment is conducted according to the results of susceptibility tests.The effective antimicrobial drugs to infections caused by ESBLs-producing bacteria are carbapenem,cefoxitin,complex antibiotics containing β-lactamases inhibitor and amikacin.