Detection and Analysis of Antimicrobial Resistance of Extendedspectrum Beta-lactamase Produced K. Pneumoniae and E. Coli

Objective:To investigate the resistance and extended-spectrumβ-lactamases (ESBLs) in E.coli and K.pneumoniae.Methods:ESBLs production was detected by CLSI/NCCLS phenotypic confirmatory test in E.coli (n=246) and K.pneumoniae (n=106).Antimicrobial susceptibility was tested by Kirby-Bauer method.Results:The positive rates of ESBLs-producing among E.coli and K.pneumoniae were 42.68% and 52.83% respectively.ESBLs producing strains in our study were highly susceptible to imipenem,and relatively susceptible to cefoperazone/sulbactam,piperacillin/tazobactam and cefoxitin.ESBLs-producers had much higher resistance rates to other antimicrobial agents compared with ESBLs non-producing strains.Conclusion:It is very important to determine ESBLs producing strains.Correctly selecting antibiotics for infection treatment is conducted according to the results of susceptibility tests.Imipenem,cefoperazone/sulbactam,piperacillin/tazobactam and cefoxitin are the effective antibiotics to infections caused by ESBLs-producing strains.