Establishment of Regeneration System of Strawberry

Taking the strawberry leaves of 'Meiho' and 'Benihope' as experimental material,we have studied the effects of different basic media,dark culture time,hormone concentration,leaf age and way of placement on inducing adventitious shoot of strawberry.The results showed that regeneration of adventitious shoots of different cultivars have different optimum conditions.The best medium of 'Meiho' was MS+2.5 mg/L TDZ+0.1 mg/L IBA+0.1 mg/L 2,4-D,the optimum leaf age was 30~40 days,the regeneration rate was up to 82.8%;the best medium of 'Benihope' was MS+2.0 mg/L TDZ+0.1 mg/L IBA+0.1 mg/L 2,4-D and the optimum leaf age was 10~20 days,the regeneration rate was 79.8%.Dark culture for 14 days could improve the regeneration rate of adventitious buds.The leaves whose abaxial surface touched the medium were better than that of inaxial surface touched the medium.Adding AgNO3 of 8 mg/L and activated carbon of 1 000 mg/L could promote the regeneration of adventitious buds.