in vitro Efficient Regeneration System of Strawberry

Six cultivars of strawberry(Fengxiang,Guilugan,Quanmingxiang,Zhangji,Hali,Xingxiang)were investigated for factors affecting the regeneration of adventitious bud.The results indicated that the genotype,hormones and seedling ages were the main factors affecting regeneration.The optimum shoot inducing medium of the leaves from Guilugan was MS+2.0 mg/L 6-BA+0.1 mg/L IBA,the better medium for the induction of callus from the leaves of Zhangji was MS+mg/L 6-BA3+0.2 mg/L 2,4-D;the optimum medium for shoot elongation was MS+0.5 mg/L 6-BA+0.5 mg/L IBA,the optimum root inducing medium were MS+0.2 mg/L IBA.The survival rate of the plantlets transplanted was 87%.Dark incubation for 1 week can reduce the browning of cultures.A highly efficient in vitro regeneration system from leaf explants was established.